Fig. 4.
Effect of acute hypoxia and TPA on secreted vIL-6 production and intracellular PF-8 expression from KSHV-infected BCBL-1 cells.
BCBL-1 cells were seeded at 300 000 cells per milliliter and then incubated in normoxia or hypoxia (1% O2). Following incubation overnight, cells were either left untreated or treated with TPA (6.4 nM) and returned to their respective conditions. (A) After incubation for 72 hours, the cells were harvested and analyzed for vIL-6 in the supernatant. (B) PF-8 (▪) and gpK8.1 (▨) lytic protein expression in the viable cells.
