Stimulator activity of cultured CD4⁺/HLA-DR^hi and CD4⁺/HLA-DR^lo human DC precursors in MLR.

Cells in the CD4⁺/HLA-DR^hi fraction (▴) and the CD4⁺/HLA-DR^lo fraction (▿) were cultured under the same conditions described in Figure 1B and analyzed for their stimulatory activity to allogeneic human T cells. Freshly isolated human peripheral blood mononuclear cells (○) and allogeneic CBMNC (♦) were also used as MLR stimulator cells. Two replicate experiments yielded similar results. CPM indicates counts per minute.