Effect of proteasome inhibitors on steady-state levels of MIP-1α, MIP-1β, and IL-8 mRNAs induced by anti-CD11b/c mAbs or sCD23 fusion proteins.
(A) Nonadherent monocytes (7 × 106 cells) were not preincubated or were preincubated with 40 μM PSI for 1 hour and then stimulated for 30 minutes with medium alone, anti-CD11a (5 μg/mL), anti-CD11b (5 μg/mL), or anti-CD11c (5 μg/mL) mAbs. (B) Cells were pretreated with increasing amounts of ALLN (2, 20, and 100 μM) for 1 hour and then stimulated with anti-CD11b (5 μg/mL) or anti-CD11c (5 μg/mL) mAbs for 30 minutes. (C,D) Monocytes were not preincubated or were preincubated for 1 hour with 40 μM PSI or 100 μM ALLN before stimulation for 30 minutes with ZZ-CD23 (2 μg/mL), MBP-CD23 (1 μg/mL), or medium alone. Total RNAs were isolated and analyzed by RPA. Results are representative of 3 similar experiments.
