Engagement of CD11b and CD11c by mAbs or sCD23 fusion proteins rapidly stimulates secretion of MIP-1α and MIP-1β on freshly isolated human monocytes.
(A) Human monocytes (50 × 103 cells/well) were incubated for 7 hours in medium alone or in the presence of the following antibodies or recombinant fusion proteins: isotype control IgG1 (5 μg/mL), anti-CD11a (clone BU17, 5 μg/mL), anti-CD11b (clone ICRF44, 20 μg/mL), anti-CD11c (clone BU15, 5 μg/mL), ZZ-CD23 and MBP-CD23 (0.5 μg/mL), and ZZ–P selectin (5 μg/mL). Secreted MIP-1α and MIP-1β in the culture supernatants were detected by ELISA. Data are mean ± SE values (n = 3). Similar results were obtained with anti-CD11a (clone G43-25B), anti-CD11b (clone 44), and anti-CD11c (clone 3.9) mAbs (data not shown). (B) Monocytes were cultured for various times under the same conditions as the experiment depicted in panel A. Then, MIP-1α and MIP-1β were detected in the culture supernatants by ELISA. Data are mean ± SD values from triplicate performance of one experiment representative of 2 others (for each condition, SD was < 5%).
