Fig. 1.
Time course of MIP-1α and MIP-1β mRNA induction by β2 integrin engagement.
Nonadherent human monocytes (7 × 106 cells) were left untreated or stimulated for various times with the following effectors: anti-CD11b (ICRF44, 2 μg/mL; A) anti-CD11c (BU15, 2 μg/mL; B), anti-CD11a (BU17, 2 μg/mL; C) MBP-CD23 (1 μg/mL; D) ZZ-CD23 (1 μg/mL; E), and ZZ–P selectin (5 μg/mL; F). Cells were then harvested and total RNA was isolated and analyzed by RPA for expression of MIP-1α, MIP-1β, IL-8, and GAPDH mRNAs. Results are representative of 2 distinct experiments.
