Fig. 8.
SDF-1 affects the RGS18 binding to Gαi.
ES cell–derived megakaryocytes were stimulated with SDF-1α (lanes 5-8) for 15 minutes (lane 5) or 60 minutes (lane 6) in the presence of TPO (lanes 3, 4, 7, 8) for 15 minutes (lanes 3, 7) or 60 minutes (lanes 4, 8) or in the absence of TPO (lanes 2, 5, 6, 9, 10), and the cell lysates were prepared. In vitro bindings of Gαi (A) or Gαq (B) to GST-RGS18 were performed with the cell lysates, and the bound proteins were blotted with anti–Gαi-3 (A) or anti-Gαq/11 (B) antibody. Arrowheads indicate the bound Gα. The bindings of RGS18 to Gαi or Gαq with (lane 10) or without (lane 9) AlF4−treatment are shown as controls.
