RGS18 expression in hematopoietic cells.
The fixed suspension cells isolated from mouse bone marrow (A) and the fixed section of mouse spleen (B) were stained with anti-RGS18 rat antibody followed by Cy3-conjugated F(ab′)2 fragment goat antirat antibody, together with DAPI. RGS18 is seen in red and chromosomes are seen in blue. The Hoechst−Rhodamine− cells/SP cells as hematopoietic stem cells (C), Ly-6G/Gr-1+ or CD11b/Mac-1+ cells as granulocyte and monocyte mix (D), and TER-119+ cells as erythrocyte and megakaryocyte mix (E, F) were isolated from mouse bone marrow by fluorescence-activated cell sorting and were immunostained with a purified FITC-labeled anti-RGS18 antibody (C-E). The TER-119+ cells were stained with FITC-labeled anti-RGS18 antibody (E) and with DAPI (F).
