Expression of RGS18 mRNA in mouse tissues and hematopoietic cells.

The filters were hybridized with the DIG-labeled RGS18 cDNA probe. (A) Northern blot analysis of RGS18 in various mouse tissues. Two micrograms of polyA⁺ mRNAs from the mouse tissues were loaded on the blots: brain (lane 1), heart (lane 2), kidney (lane 3), liver (lane 4), lung (lane 5), skeletal muscle (lane 6), skin (lane 7), small intestine (lane 8), spleen (lane 9), stomach (lane 10), testis (lane 11), and thymus (lane 12). Sizes of mRNA standards in kilobases (kb) are shown at left. (B) Detection of RGS18 by RT-PCR in various mouse tissues. The cDNAs prepared from various mouse tissues were used as templates for PCR. Upper panel: RGS18; lower panel: G3PDH. PCR was performed in 34 and 38 cycles for RGS18, and 22 and 26 cycles for G3PDH. Heart (lane 1), brain (lane 2), spleen (lane 3), lung (lane 4), liver (lane 5), skeletal muscle (lane 6), kidney (lane 7), testis (lane 8), 7-day embryo (lane 9), 11-day embryo (lane 10), 15-day embryo (lane 11), and 17-day embryo (lane 12). (C) Northern blot analysis of various mouse hematopoietic cell lines. Five micrograms of polyA⁺mRNAs were loaded on the blots: Ba/F3 cells (lane 1), CTLL-2 cells (lane 2), WEHI-3B cells (lane 3), FD-TPO cells (lane 4), and SKT6 cells (lane 5). Equal loading and integrity of the mRNA samples was confirmed by ethidium bromide staining of the ribosomal RNA bands (bottom panel).