Fig. 3.
Fig. 3. Western blot analysis for BOB.1/OBF.1 and Oct2. / Expression of BOB.1/OBF.1 and Oct2 was analyzed with 50 μg whole cell protein extracts; RelA was investigated in parallel for control. There was no expression of Oct2 and BOB.1/OBF.1 in the HD-derived cell lines KMH-2, L428, and L1236, whereas Raji and Namalwa (B-cell control cell lines) were clearly positive. The presence of RelA demonstrates equal amounts of intact protein in all cell lines.

Western blot analysis for BOB.1/OBF.1 and Oct2.

Expression of BOB.1/OBF.1 and Oct2 was analyzed with 50 μg whole cell protein extracts; RelA was investigated in parallel for control. There was no expression of Oct2 and BOB.1/OBF.1 in the HD-derived cell lines KMH-2, L428, and L1236, whereas Raji and Namalwa (B-cell control cell lines) were clearly positive. The presence of RelA demonstrates equal amounts of intact protein in all cell lines.

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