Effects of long-term treatment with AS-ODN.

Cell proliferation (A), metabolic activity (B), and death index (C) after long-term incubation with various AS-ODN treatments. Cells were exposed to 1 nM of the following treatments: (▪), ASH-ODN; (▴), ASL-ODN; (○), IN-ODN; (▵), untreated cells; (⋄), ASH-ODN + ASL-ODN; +, DFO 50 μM for 24 hours. Relative metabolic activity was calculated using the fluorescence (Fl) of Alamar Blue–treated cells (see “Materials and methods”) and according to the formula (Fl − Fl_CHX)/(Fl_Untr − Fl_CHX), where CHX is 5 μM cycloheximide and Untr is untreated cells. Data are from a representative experiment performed in triplicate.