Tf-⁵⁵Fe uptake and transferrin receptor (TfR) surface expression in cells treated with AS-ODN against FT.

(A) Tf-⁵⁵Fe uptake. (B)Transferrin receptor (TfR) surface expression. After incubation with various AS-ODNs, uptake of Tf-⁵⁵Fe and surface expression of TfR were assayed, as described in “Materials and methods.” Treatments in panel A were none (▿), IN-ODN (○), ASH-ODN (▪), ASL-ODN (▴), ASH-ODN + ASL-ODN (⋄), and ferric ammonium citrate (20 μM, 24 hours) (X). TfR surface expression in all treatments besides IN-ODN were significantly different (P ≤ .05) in the untreated cells, as determined by independent t test. Data are from a representative experiment performed in triplicate.