Cytokine stimulation-induced homing of CD34⁺CD38⁺ cells.

(A) G-SCF MPB CD34⁺-enriched cells were incubated with SCF (50 ng/mL) for 3.5 days. Flow cytometry analyses of CD34/CXCR4 (i) and CD34/CD38 (ii) staining before and after 3.5 days of stimulation are shown. Homing CD34⁺CD38⁺ cells in the BM of transplanted mice (iii). A representative experiment of 3 performed is shown. (B) CD34/CXCR4 (i) and C34/CD38 (ii) status of purified CB CD34⁻CD38⁻Lin⁻ cells. Enriched CD34⁻CD38^−/low and CD34⁺CD38^−/low cells before transwell migration (iii, see also Table 1).