¹²⁵I–IL-13 binding to T98G cells transfected with IL-13Rα1 or IL-13Rα2 chains.

cDNA for receptor chains (6 μg/chain) was transfected into T98G cells (1 × 10⁶) by using GenePORTER reagent for 48 hours. For the IL-13 binding assay, 1 × 10⁶ cells were incubated with 200 pmol/L of ¹²⁵I–IL-13 with or without a 200-fold molar excess of unlabeled IL-4 or IL-13 (A). Displacement curve (B) and Scatchard data (C) with T98G cells transfected with IL-13Rα2 chain were analyzed by the LIGAND program.