Phenotypic expression of cell surface molecules at different time points.

(A) Expression of CD3, CD8, CD4, αβTCR, and γδTCR from cells initiated from splenocytes (day 0) and at days 7, 14, and 21 in culture. (B) Expression of the activation markers CD69, CD25, CD44, and B220 at day 0 and at different time points. (C) Expression of the NK markers DX-5 and NK1.1 at day 0 and at different time points. Background staining using an isotype control antibody is shown in each histogram (dotted line). Results are representative of more than 3 experiments.