Specificity of each peptide-induced monocyte chemotaxis.

Monocytes were pretreated with HFYLPM (10 μM), MFYLPM (10 μM), or HFYLPm (10 μM) for 10 minutes at 37°C, washed twice, and thereafter allowed to migrate toward the maximal effective concentrations of each peptide (1 μM HFYLPM, 1 μM MFYLPM, or 10 nM HFYLPm) or medium. After fixing and staining of the membrane, migrated cells were quantified microscopically. The data are presented as mean ± SE of 3 independent experiments each performed in duplicate. *P < .01 versus medium treatment, †P < .01 versus peptide treatment, respectively.