Fig. 5.
![Fig. 5. Differentiation specificity of the peptides. / HL60 cells were cultured in RPMI medium containing 20% fetal calf serum. To induce differentiation of the cells into granulocytes, the cells were cultured in the presence 1.25% DMSO for 4 days. Healthy HL60 (■) and differentiated HL60 cells (dHL60, ▪) were loaded with fura-2 and treated with various concentrations of HFYLPM (A), MFYLPM (B), and HFYLPm (C). The peak level of the [Ca++]i increase was recorded. The data are means ± SE from 4 independent experiments. *P < .01 versus vehicle treatment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2854/6/h80910966005.jpeg?Expires=1724240272&Signature=d9FNYa5ypo5F06e8Ti6WC4PVucz4igUgkoTH2m3bQwEqPhBjfKY2w5Rq93vuUC9tlx7gDjNLXjrlcsHFwuc48PaqlAc0d~WhyA99kkvxQTv6uYdzPc2tt7MHQsEPMzwv~Z3RlufmpDCtvByzZYgj7zdbO4aKph81RV0dkfAyChlDJHg80ovvO1dZVrmmuybqAjwXJJnlC0WVYr3tT6kapU67knPNdVM1Lw6p865Ld4Qz8X7LCQjD0WRVX7yn6zaX6PXfPhJdeTqJGxG8qRnEmzEA3aFRfMxeH4jlmAS0OowT257M0vfOxEyvjyWQ-GmdmID9y2fM9JpFoCLHImvyng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Differentiation specificity of the peptides.
HL60 cells were cultured in RPMI medium containing 20% fetal calf serum. To induce differentiation of the cells into granulocytes, the cells were cultured in the presence 1.25% DMSO for 4 days. Healthy HL60 (■) and differentiated HL60 cells (dHL60, ▪) were loaded with fura-2 and treated with various concentrations of HFYLPM (A), MFYLPM (B), and HFYLPm (C). The peak level of the [Ca++]i increase was recorded. The data are means ± SE from 4 independent experiments. *P < .01 versus vehicle treatment.
