Fig. 4.
![Fig. 4. Cell specificity of peptides. / Prepared human primary monocytes, neutrophils, and cultured HL60, Jurkat, U937, NIH3T3, 3Y1, 3T3L1, and PC12 cells were loaded with fura-2 and stimulated with effective concentrations of the 3 peptides (1 μM for monocytes and neutrophils; 10 μM for HL60 and Jurkat; 20 μM for U937, NIH3T3, 3Y1, 3T3L1, and PC12). (A) The peak level of the [Ca++]i increase was recorded. (B) Monocytes and neutrophils were stimulated with 10 μM of each peptide. Superoxide generation was measured using cytochrome c reduction assay. Data are presented are means ± SE from at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2854/6/h80910966004.jpeg?Expires=1724240030&Signature=GWaSDUaw0zglzYdKtq9EYxcTw9CI1~PMNELq~AsQTA2Vevff5PLAdNx0JtQWiMDjV4qdRygAmw6kyBxPwRPZjYE-UnWjnumZjeG-PbUMgpm2ihID4VVG~1iktBB69P4sbWp8ajd8BAFZ3~baNWY3K2icaq5Hsm-7di2SPi263n9TO6b~AufXdA~LYLoylXnm76ZRT5xEYQU8SJeIYRR9YKD7BfAJ2mdEbQhX4dDHEaCODRhYKw~7U7qn9MCuKYebx2~y8WEGIxeYGSm-4AB~V54Mj9MJZK39XK6-lYC2GuqmEqGIb3QgBBSdZKtbD7mbGCnNg0RRNuc64TUqfFJuIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cell specificity of peptides.
Prepared human primary monocytes, neutrophils, and cultured HL60, Jurkat, U937, NIH3T3, 3Y1, 3T3L1, and PC12 cells were loaded with fura-2 and stimulated with effective concentrations of the 3 peptides (1 μM for monocytes and neutrophils; 10 μM for HL60 and Jurkat; 20 μM for U937, NIH3T3, 3Y1, 3T3L1, and PC12). (A) The peak level of the [Ca++]i increase was recorded. (B) Monocytes and neutrophils were stimulated with 10 μM of each peptide. Superoxide generation was measured using cytochrome c reduction assay. Data are presented are means ± SE from at least 3 independent experiments.
