Sorting regions used for preparing Lin⁻c-kit⁺Sca-1⁺ cells of 5-FU–treated mice that were further separated on the basis of CD38 and CD34.

The Lin⁻ cells were enriched for c-kit⁺Sca-1⁺ cells using R14 window and again sorted on the basis of CD38 and CD34 expression using R15 to R18 windows. We used anti-CD38 (90) antibody from Pharmingen in this experiment.