Sorting regions used for preparing Lin⁻c-kit⁺Sca-1⁺ PB cells of G-CSF–treated mice that were further separated on the basis of CD38.

First, Lin⁺ cells were removed from mononuclear PB cells with immunomagnetic beads. The Lin⁻ cells were enriched for c-kit⁺Sca-1⁺ cells using R11 window and again sorted on the basis of CD38⁻ (R12) and CD38⁺ (R13) windows. The experiments were performed with NIM-R5 anti-CD38 antibody.