Sorting regions and flow cytometric analyses of CD38 expression before and after culture.

Top: Sorting regions used for preparing the Lin⁻c-kit⁺Sca-1⁺CD38⁺population from bone marrow cells of normal mice. The Lin⁻cells were enriched for c-kit⁺Sca-1⁺ cells using R7 windows and again sorted on the basis of CD38 expression using the R8 window. The cells were incubated with SF and IL-11 for 1 week. Bottom: Sorting regions used for the cultured cells and analyses of purity of the sorted cells. Dotted and solid lines represent cells stained with IgG2a-FITC and CD38-FITC, respectively. The anti-CD38 antibody used in this experiment was purchased from Southern Biotechnology Associates.