Expression of TER-119 and 4A5 in the light-density cells of the spleen.

(A) (B) Representative flow cytometric analysis for the expression of TER-119 (y-axes) and 4A5 (x-axes) in the light-density cells of the spleens from untreated (steady-state), day-1 PHZ-treated, and day-6 EPO-treated wild-type (panel A, top panels) and GATA-1^low(panel B, bottom panels) mice. The numbers in the quadrants of each dot plot indicate the frequency of cells with the corresponding phenotype. Similar results were obtained in 3 additional experiments. Each experiment included negative controls represented by PE- and FITC-conjugated isotype-matched irrelevant antibodies, which are not presented because they are identical to those already published.26