Immunohistochemical analysis of spleens obtained from wild-type (left panels) and GATA-1^low (right panels) mice.

(A) (B) Hematoxilin-eosin staining of paraffin-embedded sections; 10 × and 40 × magnification. (C) (D) 4A5-specific immunostaining (panel C). GATA-1–specific immunostaining (panel D). The black and white arrows identify representative GATA-1⁺ or GATA-1⁻ megakaryocytes, respectively, while the small arrows identify representative GATA-1⁺ erythroblasts. The small insert in the GATA-1^low panel shows the GATA-1 staining of a splenic section from a representative heterozygote female, as a control. There is 40 × magnification in all cases.