Trx80 induces production of IL-12 from CD40⁺monocytes in PBMC.

Human PBMC were cultured in AIM V medium in ultralow attachment plates for 48 hours. Twenty-two hours before terminating the cultures, Brefaldin A was added to each culture. After harvesting, the large cell population in PBMC was analyzed for intracellular expression of IL-12 and expression of CD40. The culture conditions were unstimulated cells (A), Trx80 1 μM (B), IL-2 at 20 U/mL (C), Trx80 1 μM and IL-2 at 20 U/mL (D), LPS 100 ng/mL (E), and unstimulated cells with isotype-matched antibodies for IL-12 and CD40 (F). One representative experiment of 4 is shown. The proportion of cells positive for both CD40 and IL-12 is indicated in upper right corner.