Endotoxin inhibitor PMB inhibits IL-12 secretion induced by LPS but not by Trx80.

Human PBMC were stimulated for 3 days with Trx80, LPS, or PMB alone and together in AIM V medium. Subsequently cell medium was collected and analyzed for IL-12 content with sandwich ELISA, which detects both p40 and IL-12 heterodimer with a detection limit of 0.3 ng/mL. Bars indicate means from duplicate measurements in sandwich ELISA. Figure shows one representative experiment of 3.