Kinetics of IL-12 secretion from purified human PBMC stimulated with Trx80 and IL-2.

Human PBMC were stimulated with Trx80 alone or together with IL-2 in AIM V medium. Cell medium was collected after 1, 2, and 3 days and subsequently analyzed for IL-12 content using a sandwich ELISA that detects both p40 and IL-12 heterodimer with a detection limit of 0.3 ng/mL. Trx80 at 1 μM is indicated in open diamonds, 100 nM Trx80 together with 20 U/ml IL-2 in black squares, and 1 μM Trx80 together with 20 U/ml IL-2 in black diamonds. The values shown are means from duplicate measurements in sandwich ELISA. The figure shows one representative experiment of 3.