Trx80 induces secretion of IL-12 from human PBMC.

Human PBMC were stimulated with Trx, Trx80, and IL-2, alone and together. PBMC were stimulated for 3 days in AIM V medium and subsequently cell medium was collected and analyzed for IL-12 content with sandwich ELISA, which detects both p40 and IL-12 heterodimer with a detection limit of 0.3 ng/mL. The figure shows one representative experiment of 9 and the bars depict the mean of duplicate measurements in sandwich ELISA.