Immunoblot analysis of XIIIA and XIIIB in patients' plasma.

Plasma samples were diluted at ratios of 1:5, 1:10, 1:20, and 1:40 for XIIIA, and 1:20, 1:40, 1:80, and 1:160 for XIIIB from the left to the right. Each sample was subjected to 8% SDS-PAGE under nonreducing conditions and immunoblot analysis using an anti-XIIIA or anti-XIIIB antibody.