IFN-α does not induce CD95L mRNA expression.

Daudi cells were stimulated as indicated for 20 hours. Jurkat cells, which were cultured with medium or coated CD3 monoclonal antibody for 4 hours, were used as positive control (Co). mRNA levels of CD95L and β-actin were assessed by RT-PCR, and PCR products were visualized after electrophoresis on a 1% agarose gel.