Subcellular localization of GABPα, GABPβ, +-MITF, or

mi-MITF examined by immunocytochemistry. (A) GABPα or GABPβ was expressed alone, or GABPα and GABPβ were coexpressed. Cells were stained with either anti-GABPα Ab or anti-GABPβ Ab. (B) GABPα and +-MITF, or GABPβ and +-MITF were coexpressed. Cells were stained with the mixture of anti-GABPα Ab and anti-MITF Ab, or the mixture of anti-GABPβ Ab and anti-MITF Ab. (C) GABPα and mi-MITF, or GABPβ and mi-MITF were coexpressed. Cells were stained with the mixture of anti-GABPα Ab and anti-MITF Ab, or the mixture of anti-GABPβ Ab and anti-MITF Ab (original magnification × 2000).