The effect of coexpression of +-MITF or

mi-MITF cDNA on the luciferase activity. The reporter plasmids were transfected to MST cells with the expression plasmid containing +-MITF or mi-MITF cDNA (5 or 10 μg). The transfected DNA was always kept in equal amount using the backbone expression vector in each transfection assay. The data represent the mean ± SE of 3 experiments. In some cases, the SE was too small to be shown by bars. P < .01 by t test when compared with the luciferase activity obtained by the cotransfection of the reporter plasmid with the expression vector alone.