EGMSA using nuclear extract of MST cells.

Oligo 1 containing the intact GGAA motifs was used as a probe. As a competitor, nonlabeled oligo 1, oligo 2 mutated at the 5′-GGAA motif, or oligo 3 mutated at the 3′-GGAA motif was added. Arrows indicate the 2 specific DNA/protein complexes and the free probe. The super-shift assay was performed using the anti-GABPα, GABPβ, or Ets-1 Ab. The arrowhead indicates the super-shifted band.