DNA-binding ability of c/ebp1.

Electrophoretic mobility shift assay was performed using in vitro transcribed and translated proteins and a ³²P-labeled C/EBP optimal binding site probe. (A) Unlabeled in vitro translation control protein (luciferase) or c/ebp1 was incubated with a³²P-labeled C/EBP site probe and, where indicated, with 50-fold excess of unlabeled competitor self probe (WT) or mutant probe (M) and samples were then separated on a 4% TBE gel. The gel was dried and autoradiography was performed. (B) ³⁵S-labeled in vitro translation control protein (luc) and c/ebp1 were separated on a 4% to 12% bis-tris gradient gel. The processed gel was then exposed to film for autoradiography. Size markers of 62 kd and 28 kd are indicated.