Analysis of c/ebp1 and l-plastinexpression in hematopoietic mutant embryos.

RNA in situ hybridization with c/ebp1 andl-plastin RNA antisense probes using wild-type,cloche, and m683 embryos at 24 hpf. Stained cells are seen over the yolk sac in wild-type and m683 embryos. Individual sampled embryos have variable staining patterns with a single probe (either c/ebp1 or l-plastin) because the stained cells were freely flowing over the yolk sac and in the circulation before sacrifice and fixation of the embryos. Lack of staining using c/ebp1 or l-plastin probes was observed in 25% of the cloche embryos from at least 3 independent heterozygote in-crosses.