Alignment and phylogenetic tree of c/ebp1with human C/EBP family members.

(A) Zebrafish (Dr) c/ebp1 was aligned with known human (Hs) C/EBP sequences using CLUSTAL within the GCG program (http://molbio.info.nih.gov/molbio/gcglite/). Black shading shows identities and gray shading shows conserved amino acid substitutions seen in at least 3 of 7 sequences. The leucines that make up the leucine zipper in c/ebp1 are marked with asterisks and the additional leucine present in the leucine zippers of C/EBPα, β, and δ is marked with a filled circle. The bZIP domain5 of c/ebp1 is underlined and the region encoded by the probe used to screen the zebrafish cDNA library is delineated with arrows. The GenBank accession number is AF306857. (B) A phylogenetic tree was generated from an alignment of the bZIP regions of the zebrafish (Dr) c/ebps and the human (Hs) C/EBPs using CLUSTAL within the DNAstar Megalign program.