FVIII-specific cytokine production is inhibited after blocking CD40-CD40L interaction in vivo.

Peritoneal macrophages and T cells from 3 fVIII-injected (■) and 3 anti-CD40L plus fVIII-treated mice (♦) were pooled and cultured with or without fVIII. Culture supernatants were harvested at 2 days (IL-2) and 4 days (IL-4, IFN-γ, and IL-10), and cytokine ELISAs were performed. Results show the mean ± 1 SD of duplicates.