Histologic analysis of leukemic infiltrates in engrafted NOD/SCID mice.
Tissue sections (A-E) were obtained from a mouse preconditioned with 25 × 106 cord blood MNCs 7 days before the injection of 36 × 106 primary T-ALL cells. The mouse was killed 40 days after the T-ALL injection. Tissues were fixed in aqueous-buffered zinc formalin and embedded in paraffin; 4-μm sections were prepared and stained with hematoxylin and eosin. (A) Liver, (B) kidney, (C) adrenal gland, (D) lymph node, and (E) thymus from the engrafted mouse. For comparison, a tissue section (F) was obtained from the normal thymus of an unmanipulated, age-matched NOD/SCID mouse. Human T-ALL engraftment levels in bone marrow and spleen for this mouse were found by flow cytometry to be 85% and 78%, respectively (not shown).
