Characterization of DCs in bone marrow allografts.

(A) Frequencies of cells with phenotypes (1) CD3⁻, CD4^bright, CD8⁻, low side-scatter, (2) Lin⁻, CD123^bright, HLADR⁺, CD11c⁻, and (3) Lin⁻, CD11c⁺, HLADR⁺ were determined by FACS analysis of 29 samples of bone marrow harvested from healthy allogeneic donors. The number of donor cells/kg (×10⁶) in the allograft with phenotype 1 was compared to the numbers of cells with phenotype 2 (○) or 3 (▪). Equations for the best-fit lines comparing the relation between populations 1 and 2 (slope, 1.2; r = 0.92) and between populations 1 and 3 (slope, 2.7; r = 0.85) are shown. (B) Ability of unfractionated bone marrow cells (○) and FACS-isolated CD4⁺ T-cells (⋄). FACS-isolated CD123⁻ cells (▵) and FACS-isolated Lin⁻, CD4^bright, CD123^bright cells (♦) to stimulate proliferation of allogeneic lymphocytes in an MLR was assessed after 5 day co-culture in media containing 10% AB⁻ human serum, 50 ng/mL IL-3, and 100 ng/mL GM-CSF.