RELT-hFc fusion protein and its expression.

(A) Diagram of RELT-hFc fusion protein construct. ECD is the extracellular domain of RELT that is linked to the Fc portion of hIgG1. ARR/GV is the amino acid sequence of the proposed cleavage site for the truncated form of the soluble protein. (B) Western blot analysis of recombinant RELT-hFc fusion protein from transfected 293 supernatant. After SDS-PAGE and transfer to nitrocellulose filter paper, fusion protein was detected with a goat antihuman Fc specific HRP-conjugated polyclonal antibody.