Schematic representation and binding properties of the MC and FCR3-CSA CIDR-1 chimeras.

MC and FCR3-CSA CIDR-1 sequences that were re-engineered with restriction sites and various chimeras between them were tested for binding to CD36. The various constructs were transfected into COS-7 cells and tested for binding to CD36-coated Dynal beads as in Figure 1. The percentage of cells with 4 or more beads attached is given. In each assay, 100 cells expressing the recombinant protein, as determined by immunofluorescence, were examined. Results are expressed as the mean and SD of 3 independent experiments.