Serum TNF levels are not significantly increased in any of the transplantation groups.

All C3FeB6F1 recipients underwent transplantation, as illustrated in Figure 1, and serum was obtained on day 14 or 15 after BMT. Serum TNF levels were determined by ELISA, as described in “Materials and methods.” Mean serum TNF level (±SEM) for each group is depicted, and no statistically significant differences between groups were observed. Combined data consisted of 7 to 11 sera per group, and these samples were collected from 3 different BMTs. ELISA analysis was repeated 3 times in duplicate, and results from a representative analysis are shown.