Effect of cytoskeletal modifiers on fibrinogen-induced inhibition of store-mediated Ca⁺⁺ entry.

Fura-2–loaded human platelets were preincubated in the presence of 10 μM CD for 40 minutes, 10 μM JP for 60 seconds, both agents, or the vehicles. Cells were then stimulated in a Ca⁺⁺-free medium (100 μM EGTA) with ΤG (200 nM) in the absence (Control) or presence of 1 mg/mL fibrinogen (FG, FG + CD, FG + JP, and FG + CD + JP). Three minutes later, CaCl₂ (final concentration, 300 μM) was added to the medium to initiate Ca⁺⁺ entry. Traces shown are representative of 4 independent experiments.