Fig. 1.
![Fig. 1. Fibrinogen inhibits ADP-evoked elevations in [Ca++]i. / (A) Fura-2–loaded human platelets suspended in an HBS containing 1 mM Ca++ were stimulated with ADP (5 μM) in the absence (Control) or presence of various concentrations of fibrinogen (FG; 0.1-1 mg/mL). [Ca++]i was monitored as described in “Materials and methods.” (B, C) Human platelets were stimulated with ADP (5 μM) in the absence (Control) or presence of either fibrinogen alone (FG; 1 mg/mL) or fibrinogen (1 mg/mL) in combination with RGDS (100 μM) (FG + RGDS; B) or abciximab (5 μg/mL) (ReoPro; FG + Ab; C). Traces shown are representative of 5 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/9/10.1182_blood.v97.9.2648/6/h80910986001.jpeg?Expires=1724269088&Signature=yN3~gci~eRVQ1x03uhukOE90joVsEFTfJ0h4UEnhvvPF7~dvz0VFbNUBo~V8eQCBvL5yxF2F2xhqlgQCNP~yzfw7KUuUq8IXca6pYks7O5MzEEBRjXtJZOSQtAHqoVDfSavG6ZxVoMTtYz3o70kIK6iQPowZj7MErlkDW6NmwX7d3DIO~A9314hIiwLo06MVPwoNpr93MURgr9tUqQBQbQwCqShY6jnDBbNj8RAzwGISt5cHO9Loq~qkFgbRfznor4sC01udBrjJs-NnRWBnm~IJMbDoGau5o~iRk0Rgbit~KugFOfaP5xxpONX4CnOm9d1ss2RJoG5CrJepRVPg2A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fibrinogen inhibits ADP-evoked elevations in [Ca++]i.
(A) Fura-2–loaded human platelets suspended in an HBS containing 1 mM Ca++ were stimulated with ADP (5 μM) in the absence (Control) or presence of various concentrations of fibrinogen (FG; 0.1-1 mg/mL). [Ca++]i was monitored as described in “Materials and methods.” (B, C) Human platelets were stimulated with ADP (5 μM) in the absence (Control) or presence of either fibrinogen alone (FG; 1 mg/mL) or fibrinogen (1 mg/mL) in combination with RGDS (100 μM) (FG + RGDS; B) or abciximab (5 μg/mL) (ReoPro; FG + Ab; C). Traces shown are representative of 5 independent experiments.
