Fig. 6.
Fig. 6. Kinetics and dose-response of PD98059-induced cell-cycle arrest and down-regulation of survivin protein expression. / OCI-AML3 cells were treated with either vehicle (control) or PD98059, harvested and stained with PI to determine DNA content (left panels) or analyzed by Western blot with a survivin-specific antiserum (right panels). Top panels show the kinetics of cell-cycle arrest and down-regulation of survivin expression in response to 20 μM PD98059. Bottom panels show the dose-response curves for PD98059 at 48 hours. Results are expressed as the percentage of cells in S phase (calculated using the ModFit LT software, left panels) andsurvivin/β-actin ratios (as quantitated by a PhosphorImager, right panels). Results of 1 of 3 independent experiments are shown. ○ indicates control; ●, PD98059 (20 μM).

Kinetics and dose-response of PD98059-induced cell-cycle arrest and down-regulation of survivin protein expression.

OCI-AML3 cells were treated with either vehicle (control) or PD98059, harvested and stained with PI to determine DNA content (left panels) or analyzed by Western blot with a survivin-specific antiserum (right panels). Top panels show the kinetics of cell-cycle arrest and down-regulation of survivin expression in response to 20 μM PD98059. Bottom panels show the dose-response curves for PD98059 at 48 hours. Results are expressed as the percentage of cells in S phase (calculated using the ModFit LT software, left panels) andsurvivin/β-actin ratios (as quantitated by a PhosphorImager, right panels). Results of 1 of 3 independent experiments are shown. ○ indicates control; ●, PD98059 (20 μM).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal