Regulation of survivin expression in OCI-AML3 cells by MEK and PI3K inhibitors.

(A) Western blot shows survivin protein expression in cells treated with 20 μM PD98059 (PD) or 10 μM LY294002 (LY) for 48 hours without or with GM-CSF (100 U/mL). Results of 1 of 3 independent experiments are shown. (B) Quantitative RT-PCR demonstrates the regulation of survivin mRNA expression in response to the inhibitors at 2 and 24 hours (Taqman PCR; see “Materials and methods”). ■ indicates control; ▨, PD; and ▪, LY.