ATRA-induced down-regulation of survivinprotein expression in HL-60 and OCI-AML3 cells.

HL-60 (A) and OCI-AML3 (B) cells were treated with 1 μM ATRA for up to 96 hours, as described in “Materials and methods.” Cells were lysed, and survivin protein levels were determined at 24, 48, 72, and 96 hours by Western blot analysis. The experiment was performed 3 times, and the results shown here are representative.