Effect of cytokines on survivin expression in primary AML blast cells.

Cells were cultured in serum-free AIM-V medium with GM-CSF (100 U/mL), G-CSF (50 ng/mL), SCF (100 ng/mL), or various combinations of these cytokines for 48 hours. Then they were lysed, and survivinprotein levels were assessed by Western blot analysis. Results were quantitated by PhosphorImager. GM, GM-CSF; G, G-CSF; and Pt No., patient number as shown in Table 1.