HER-2/neu-specific CTL responses induced with peptide-pulsed DCs.

In vitro generated DCs were pulsed with the E75 synthetic peptide derived from Her-2/neu and used as APC to induce a primary MHC class I restricted CTL response in vitro. Control antibody (A), SIRPβ-specific MoAb B1D5 (C), and the inhibitory MoAbs against SIRPα/β (SE5A5) (E) and CD47 (G), as well as soluble proteins (control, B), soluble SIRPβ1ex (D), SIRPα1ex protein (F) were added to the cultures during the in vitro priming. Cytotoxic activity of induced CTL was determined after 2 restimulations in a standard⁵¹Cr-release assay using Croft cells pulsed with the cognate E75 peptide (▪) or irrelevant M1.1 peptide (■) as targets.