![Fig. 2. Mac-1 is crucial for FcR-mediated PMN cytotoxicity of tumor targets. / (A) Lysis of human breast carcinoma (SK-BR-3) cells mediated by Tg Mac-1+/− PMNs (▪) and Tg Mac-1−/− PMNs (░) in the absence (control) or presence of FcαRI-directed BsAb [A77 × 520C9]. Cytotoxicity was analyzed after 4 hours at different E/T ratios (20:1 and 80:1). Results are presented as mean ± SEM (n = 6), (*P < .0001). (B) FcαRI-mediated cytotoxicity toward SK-BR-3 cells by human PMNs (E/T ratio; 50:1) in the absence (control) or presence of BsAb [A77 × 520C9]. The effect of anti–Mac-1 mAb 44a or M1/70, and NADG on ADCC was analyzed as described in “Materials and methods.” Data are presented as mean ± SEM (n = 3). *Significant difference compared with BsAb alone (P < .002).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2478/5/h80810929002.jpeg?Expires=1723712653&Signature=c-Jx7QvP3XtdGOwMxjyp4Us9ZUL78f-KteDBEAMuTOtb0wUolAKMwF34IT6~QYOwrvGpW0oR4YxU1KWo7gfI0c2m2OgLXZT6YeX80Ks2amkzcE51ccRcpHMQR0y26fyX0kScGlteMJ-mI3oHzo~WTD6I1TQnR2HkbmSt0NX7z1~yUuPEO1v~ABjDXLR9ubY8Z6ftASC6JM9jOciNt8hnWGBULUtkaD~04l40s1lg2qpKQmp9hL~0Q9-o~Ks31qMTjhbCV6r~ARDlcEeXccEoZEpf52AWMV-Huhvbzdl03~pSuMh4uqoAUosa2I6imz98hqMO~nLK-Y4vjHWWYEkKtw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mac-1 is crucial for FcR-mediated PMN cytotoxicity of tumor targets.
(A) Lysis of human breast carcinoma (SK-BR-3) cells mediated by Tg Mac-1+/− PMNs (▪) and Tg Mac-1−/− PMNs (░) in the absence (control) or presence of FcαRI-directed BsAb [A77 × 520C9]. Cytotoxicity was analyzed after 4 hours at different E/T ratios (20:1 and 80:1). Results are presented as mean ± SEM (n = 6), (*P < .0001). (B) FcαRI-mediated cytotoxicity toward SK-BR-3 cells by human PMNs (E/T ratio; 50:1) in the absence (control) or presence of BsAb [A77 × 520C9]. The effect of anti–Mac-1 mAb 44a or M1/70, and NADG on ADCC was analyzed as described in “Materials and methods.” Data are presented as mean ± SEM (n = 3). *Significant difference compared with BsAb alone (P < .002).
