Fig. 1.
![Fig. 1. Mac-1–deficient PMNs efficiently phagocytose and kill Ab-opsonized. / C albicans. Tg mouse PMNs (Mac-1+/− and Mac-1−/−) were incubated with C albicansin the presence of FcαRI-directed BsAb. (A) Phagocytosis was analyzed by light microscopy after 30 minutes. (B) Flow cytometric analysis of FcαRI-mediated C albicans uptake by Tg Mac-1+/− (▪) and Tg Mac-1−/− PMNs (░). Results are expressed as percentage of PMNs that mediated phagocytosis (mean ± SEM [n = 4]). (C) FcαRI-mediated killing of C albicans by Tg PMNs (Mac-1+/− and Mac-1−/−) after 2 hours. Results are mean ± SEM based on 4 individual experiments. Bars indicate 10 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/8/10.1182_blood.v97.8.2478/5/h80810929001.jpeg?Expires=1723714846&Signature=d8ysLi6YCahFGpGQdGxiryQV2mAbs2Glp1IehfL7ktVqtCVSB6MDPdFqm~hnL00RdGyOgy9oRslEmmPqbASC5kl3kKV~-47DJ41oY8eP5586q49Z~fulCgdioiSvv~0aXwIBDiKhqhFY0clDDjqzleWYn6gdvyFvvWi~SLbNw46cSjiOHRBAmuiI2WjI2GkfxEts8~SA-7To8tiuPL-lo3Au9xsoqbVAELTVTvO7YFYyVd8sA~WzHr4m~MbNmwhzDVGPj22gXoA7sSwtOR7moPO0CbvwqPnzFBtGH79Luht55~2ggKxG2hzL3J1VDyuCWAM3UikxLZStjB9-mQ~ZVg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mac-1–deficient PMNs efficiently phagocytose and kill Ab-opsonized
C albicans. Tg mouse PMNs (Mac-1+/− and Mac-1−/−) were incubated with C albicansin the presence of FcαRI-directed BsAb. (A) Phagocytosis was analyzed by light microscopy after 30 minutes. (B) Flow cytometric analysis of FcαRI-mediated C albicans uptake by Tg Mac-1+/− (▪) and Tg Mac-1−/− PMNs (░). Results are expressed as percentage of PMNs that mediated phagocytosis (mean ± SEM [n = 4]). (C) FcαRI-mediated killing of C albicans by Tg PMNs (Mac-1+/− and Mac-1−/−) after 2 hours. Results are mean ± SEM based on 4 individual experiments. Bars indicate 10 μm.
