Fig. 2.
Western blot analysis of cells and cell lines of B-CLL patients.
B-CLL cells were lysed and analyzed by SDS-PAGE and Western blotting using anti-Survivin mAb (top). Anti–β actin polyclonal antiserum (bottom) was used as control. Samples at diagnosis from 1 Survivin− (patient 3) and 2 Survivin+(patients 5 and 8) B-CLL patients are shown. Mec1 and Mec2 cell lines, grown in log phase, were used as controls. 3 × 106 cell equivalents per test were used.
